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KMID : 0357920060400030176
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Korean Journal of Pathology
2006 Volume.40 No. 3 p.176 ~ p.184
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Genetic Analysis of Epstein-Barr Virus Latent Membrane Protein 1 and Immunohistochemical Expression of Transforming Growth Factor (TGF)-β1, TGF-βRII, p21, p16, E2F1, Thymidylate Synthase, and NF-κB in Epstein-Barr Virus Encoded RNA-positive Gastric Adenoc
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Cho Mee-Yon
Kim Dae-Sung
Choi Sun-Ju
Eum Min-Seob
Kim Myoung-Soo
Park Kwang-Hwa
Kim Mee-Dong
Sung Seung-Hoon
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Abstract
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Background: Although clinicopathologic differences have been described between Epstein-Barr virus (EBV)-positive and negative gastric adenocarcinomas, the pathogenetic basis for these differences remains unclear. In this study, efforts were made to confirm that expression of EBV-latent membrane protein (LMP1) and immunohistochemical characteristics of EBV-positive gastric adenocarcinomas.
Methods: We investigated genomic deletion, and RNA & protein expression of the EBV-LMP1, as well as immunohistochemical protein expression of transforming growth factor (TGF)-β1, TGF-βRII, p21, p16, E2F1, thymidylate synthase, and NF-κB in relation to EBV positive gastric adenocarcinoma.
Results: A total of 38 Epstein-Barr Virus Encoded RNA-positive and 80 negative gastric carcinomas were examined. A 30 bp DNA deletion in the EBV-LMP1 gene, initiating at codon 342, was detected in 94.4% of EBV-positive cases. By RT-PCR and western blotting, EBV-LMP1 mRNA and protein expressions were absent in all cases, re- gardless of DNA deletion. No significant differences in TGF-β1, TGF-βRII, p21, NF-κB, E2F1, or thymidylate synthase expression were identified. However, the decreased expression of p16 was found in 84.2% of EBV-positive carcinomas, relative to only 57.5% of EBV-negative tumors (p=0.024).
Conclusion: EBV-LMP1 DNA deletion, mRNA and protein losses are highly prevalent in EBV-positive gastric adenocarcinoma among Korean patients, along with decreased p16 expression.
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KEYWORD
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Stomach neoplasms, Epstein-Barr virus, EBV-Associated Membrane Antigen, Protein p16
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